Plant Microtechnique And Microscopy

Plant Microtechnique and Microscopy PDF
Author: Steven E. Ruzin
Publisher: Oxford University Press, USA
ISBN: 9780195089561
Size: 30.67 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 322
View: 6469

Get Book

Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.

Plant Microtechnique And Microscopy

Plant Microtechnique and Microscopy PDF
Author: Steven E. Ruzin
Publisher: Oxford University Press, USA
ISBN: 9780195089561
Size: 53.74 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 322
View: 2258

Get Book

Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.

Plant Microtechniques And Protocols

Plant Microtechniques and Protocols PDF
Author: Edward Chee Tak Yeung
Publisher: Springer
ISBN: 3319199447
Size: 55.81 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 576
View: 6146

Get Book

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Fluorescent Proteins

Fluorescent Proteins PDF
Author: Kevin F. Sullivan
Publisher: Elsevier
ISBN: 9780080557243
Size: 69.13 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 660
View: 1423

Get Book

This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.

Biermann S Handbook Of Pulp And Paper

Biermann s Handbook of Pulp and Paper PDF
Author: Pratima Bajpai
Publisher: Elsevier
ISBN: 0128142413
Size: 60.40 MB
Format: PDF, Mobi
Category : Technology & Engineering
Languages : en
Pages : 668
View: 4277

Get Book

Biermann's Handbook of Pulp and Paper: Raw Material and Pulp Making, Third Edition is a comprehensive reference for industry and academia covering the entire gamut of pulping technology. This book provides a thorough introduction to the entire technology of pulp manufacture; features chapters covering all aspects of pulping from wood handling at the mill site through pulping and bleaching and pulp drying. It also includes a discussion on bleaching chemicals, recovery of pulping spent liquors and regeneration of chemicals used and the manufacture of side products. The secondary fiber recovery and utilization and current advances like organosolv pulping and attempts to close the cycle in bleaching plants are also included. Hundreds of illustrations, charts, and tables help the reader grasp the concepts being presented. This book will provide professionals in the field with the most up-to-date and comprehensive information on the state-of- the-art techniques and aspects involved in pulp making. It has been updated, revised and extended. Alongside the traditional aspects of pulping and papermaking processes, this book also focuses on biotechnological methods, which is the distinguishing feature of this book. It includes wood-based products and chemicals, production of dissolving pulp, hexenuronic acid removal, alternative chemical recovery processes, forest products biorefinery. The most significant changes in the areas of raw material preparation and handling, pulping and recycled fiber have been included. A total of 11 new chapters have been added. This handbook is essential reading for all chemists and engineers in the paper and pulp industry. Provides comprehensive coverage on all aspects of pulp making Covers the latest science and technology in pulp making Includes traditional and biotechnological methods, a unique feature of this book Presents the environmental impact of pulp and papermaking industries Sets itself apart as a valuable reference that every pulp and papermaker/engineer/chemist will find extremely useful

Histopathology Of Seed Borne Infections

Histopathology of Seed Borne Infections PDF
Author: Dalbir Singh
Publisher: CRC Press
ISBN: 1420038176
Size: 10.27 MB
Format: PDF, ePub, Mobi
Category : Science
Languages : en
Pages : 296
View: 7138

Get Book

Histopathology of Seed-Borne Infections is the first book to provide comprehensive coverage of seed infection and disease. It includes an up-to-date account on the development and structure of seed, pointing out the structural variations in seeds of the plant families to which most crop plants belong. The text presents techniques and advice for his

Handbook Of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy PDF
Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 0387455248
Size: 51.19 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 985
View: 1277

Get Book

Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.